Computational protocol: Structure and Mechanism of Human UDP-glucose 6-Dehydrogenase

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Protocol publication

[…] Diffraction data were collected at 100 K at Swiss Light Source station X10SA or Diamond beamline I03. They were processed with MOSFLM (, ) and subsequently scaled using the program SCALA (). Structures were solved by molecular replacement using the Phaser () program and the structure of Caenorhabditis elegans UGDH (PDB code 2O3J) as the search model. The structures were manually rebuilt in COOT (), and restrained refinement with appropriate TLS groups was performed using REFMAC5 (). Data collection and refinement statistics are summarized in . […]

Pipeline specifications

Software tools iMosflm, CCP4, Coot, REFMAC5
Applications Small-angle scattering, Protein structure analysis
Organisms Homo sapiens
Diseases Neoplasms
Chemicals Glucose, NAD, Uridine Diphosphate, Glucuronic Acid