Computational protocol: Anthrax edema toxin disrupts distinct steps in Rab11-dependent junctional transport

Similar protocols

Protocol publication

[…] Imaginal discs were dissected, fixed and stained using standard procedures. Salivary glands were dissected similarly, fixed for 30 minutes, and left attached to carcasses until ready to mount in SlowFade (LifeTechnologies #S36936), using double sided tape as a spacer to prevent tissue squashing. Antibodies: rabbit anti-GFP antibody (1/500, ThermoFisher #A6455), rat-anti GFP antibody (1/500, SCBT #sc-101536), mouse anti-Rab11 (1/200, BD Biosciences #610657), mouse anti Rab11-GTP (1/100, NewEast Biosciences #26919), D-Ecad (1/500, DSHB #DCAD2). The rabbit anti-Rip11 (1/1000) was a gift from D. Ready (Purdue University) and A. Satoh (Hiroshima University, Japan), and guinea pig anti-Sec15 (1/1000) was kindly provided by Hugo Bellen (Baylor College of Medicine). Images were collected by confocal microscopy on a Leica TCS SP5. All images were acquired using a 40X or 63X objective, and all higher magnifications were obtained using a 4X digital zoom. Co-localization quantifications in used the coloc2 tool in ImageJ. […]

Pipeline specifications

Software tools Coloc, ImageJ
Applications Laser scanning microscopy, Microscopic phenotype analysis
Organisms Bacillus anthracis, Drosophila melanogaster, Homo sapiens
Diseases Drug-Related Side Effects and Adverse Reactions
Chemicals Guanosine Triphosphate