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[…] full length coding DNA sequence (CDS) of CfCYP93B was amplified using primers cdsCfCYP93BF and cdsCfCYP93BF (Table ), designed from the sequence information of 3′ and 5′ RACE amplicons. All primers were obtained from Integrated DNA Technologies, USA. Similarly 5′ RACE fragment and full length CDS were cloned for CfCYP706C. See Table for primer details., The nucleotide sequence of CfCYP93B and CfCYP706C were conceptually translated using the ExPASy translate tool (http://web.expasy.org/translate/). Using ORF Finder (http://www.ncbi.nlm.nih.gov/gorf/gorf.html) open reading frames of CfCYP93B and CfCYP706C were predicted. Predicted protein sequences of CfCYP706C and CfCYP93B were subjected to BLASTP analysis, to find out their homologs. Multiple sequence alignment of CfCYP706C and CfCYP93B with their respective homologs was carried out using CLC genomic workbench; CLC bio, a QIAGEN Company. Theoretical isoelectric point (pI) and molecular weight (Mw) of protein (http://web.expasy.org/compute_pi/) was calculated using Compute pI/Mw tool (Bjellqvist et al., , ). Transmemebrane helices and topology of proteins were predicted using HMMTOP (http://www.enzim.hu/hmmtop/; Tusnády and Simon, , ). The secondary structures of CfCYP93B and CfCYP706C were calculated using SOPMA(https://npsa-prabi.ibcp.fr/cgi-bin/npsa_automat.pl?page=/NPSA/npsa_sopma.html; Geourjon and Deléage, ). Conserved motifs and domains of CfCYP93B and CfCYP706C were assessed using CYP module of the cytochrome P450 engineering database (Fischer et al., , ; Sirim et al., , ). Phylogenetic analysis was carried out to cluster the CYP450s, isolated in this study, into their respective CYP450 families, as previously described (Awasthi et al., ). Briefly, protein sequences of four members from each of the major CYP450 families of flowering plants (CYP71-99, CYP7 […]

Pipeline specifications

Software tools BLASTP, CLC Genomics Workbench, HMMTOP
Chemicals Heterocyclic Compounds, 2-Ring, Pyrans, Anthocyanins, Heterocyclic Compounds, 2-Ring, Pyrans, Heterocyclic Compounds, 2-Ring, Pyrans, Sugar Alcohols, Mannitol, Heterocyclic Compounds, 2-Ring, Pyrans, Heterocyclic Compounds, 2-Ring, Pyrans, Heterocyclic Compounds, 2-Ring, Pyrans