|Application:||Gene expression microarray analysis|
|Number of samples:||4|
|Release date:||Nov 28 2006|
|Last update date:||Mar 16 2012|
|Computational protocol:||TM4, Spotfinder, PSORTb, Clustal W, Comprehensive Microbial Resource, MetaCyc|
The bacteria was grown in BHI broth with or without 50 ug/ml EDDHA to generate iron restriction. In the iron-restricted medium, 50 ug/ml FeCl3 was added to the medium 5 min. after the addition of EDDHA. RNA extractions were performed on three different days using the QIAGEN RNeasy Kit, and a total of four hybridization were performed. Data were acquired using a Perkin-Elmer ScanArray Express scanner, and were quantified, normalized and analysed using the SpotFinder, MIDAS and TMEV software from TIGR's TM4 Suite of Microarray Analysis softwares.
DOI: 10.1186/1471-2164-8-72call_split See protocol