Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 4
Release date: Nov 28 2006
Last update date: Mar 16 2012
Access: Public
Diseases: Pleuropneumonia
Chemicals: Iron
Computational protocol: TM4, Spotfinder, PSORTb, Clustal W, Comprehensive Microbial Resource, MetaCyc
Dataset link Complementation

Experimental Protocol


The bacteria was grown in BHI broth with or without 50 ug/ml EDDHA to generate iron restriction. In the iron-restricted medium, 50 ug/ml FeCl3 was added to the medium 5 min. after the addition of EDDHA. RNA extractions were performed on three different days using the QIAGEN RNeasy Kit, and a total of four hybridization were performed. Data were acquired using a Perkin-Elmer ScanArray Express scanner, and were quantified, normalized and analysed using the SpotFinder, MIDAS and TMEV software from TIGR's TM4 Suite of Microarray Analysis softwares.

Repositories


GEO

GSE6366

BioProject

PRJNA99583

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Contact


Vincent Deslandes

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