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Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Nov 6 2017
Last update date: Jan 15 2018
Access: Public
Taxon: Arabidopsis thaliana
Dataset link Nuclear and cellular expression data from the whole 16-cell stage Arabidopsis thaliana embryo

Experimental Protocol


We manually pollinated flowers and either isolated whole embryos (manually using an micromanipulator; cEMB) or the nuclei from embryos (using the INTACT method and a whole embryo marker; nEMB) at 16-cell stage (16C) of development (72h after pollination). Following RNA isolation, microarray-based transcriptomic profiling was performed on 3 biological replicates each.

Repositories


GEO

GSE89098

BioProject

PRJNA350284

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Contact


Joakim Palovaara

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