Computational protocol: Full genome characterization and phylogenetic analysis of hepatitis B virus in gibbons and a caretaker in Central Kalimantan, Indonesia

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Protocol publication

[…] Reference sequences were retrieved from the DDBJ, EMBL, and GenBank databases. Sequences were aligned using the Clustal X multiple sequence alignment program. Phylogenetic trees were constructed using the neighbor-joining method, and bootstrap resampling was performed 1,000 times. These analyses were carried out using the Molecular Evolutionary Genetics Analysis (MEGA) program. Subgenotypes were assigned as described previously [, , ]. […]

Pipeline specifications

Software tools Clustal W, MEGA
Databases DDBJ
Application Phylogenetics
Organisms Hepatitis B virus, Homo sapiens
Diseases Hepatitis B