Computational protocol: Crystallization and X ray data analysis of the 10 kDa C terminal lid subdomain from Caenorhabditis elegans Hsp70

Similar protocols

Protocol publication

[…] Data were collected at station 10.1, SRS, Daresbury, UK for the native data set I and station BM14, ESRF, Grenoble, France for the native data set II and the mercury-derivative crystal. All data were collected at 100 K. MAD data were collected from a single derivative crystal at two wavelengths, corresponding to the mercury L III peak (1.005 Å) and the L III-edge inflection point (1.009 Å). All data were indexed and integrated using the MOSFLM (Leslie, 1992) package and scaled using SCALA (Collaborative Computational Project, Number 4, 1994). […]

Pipeline specifications

Software tools iMosflm, CCP4
Applications Small-angle scattering, Protein structure analysis
Organisms Dipturus trachyderma, Caenorhabditis elegans, Homo sapiens, Rattus norvegicus
Chemicals Mercury