Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 32
Release date: Sep 25 2015
Last update date: Dec 28 2015
Access: Public
Diseases: Deficiency Diseases
Chemicals: Hydrogen
Computational protocol: NextGenMap, HTSeq, edgeR, VENNY, GO::TermFinder
Dataset link The Candida albicans Histone Acetyltransferase Hat1 Regulates Stress Resistance and Virulence via Distinct Chromatin Assembly Pathways

Experimental Protocol


RNA Sequencing was performed of wild type, Hat1-deficient, Cac2-deficient and Rtt109-deficient Candida albicans strains prior to and after treatment with hydrogen peroxide. Cells were grown to the exponential phase in YPD at 30oC. Aliquots were harvested for RNA isolation and to the remaining culture hydrogen eproxide was added to a final concentration of 1.6 mM. After 30 min incubation at 30°C cells were harvested for RNA isolation. Five biological replicates were done for the wild type and Hat1-deficient cells and three biological replicates were done for Cac2-deficient and Rtt109-deficient strains.

Repositories


GEO

GSE73409

ENA

SRP064163

BioProject

PRJNA296914

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