Dataset features

Specifications


Application: Gene expression microarray analysis, miRNA array analysis
Number of samples: 6
Release date: Oct 6 2011
Last update date: Mar 22 2012
Access: Public
Genes: AGO2, MIR494
Dataset link HeLa cell mitochondrial microRNAs

Experimental Protocol


To assess whether nuclear-encoded miRNA are detectable in human mitochondria, we performed the following four steps approach. First, cultured HeLa cells were allowed to reach 80-100% confluence and subjected to fractionation in order to isolate the cytosolic fraction. From the same HeLa cells, mitochondria were isolated by immunomagnetic Anti-TOM22 MicroBeads from the Mitochondria Isolation Kit (Miltenyi Biotec). In total, six mitochondria preparations were perfromed, three of these were additionally treated with RNase A. Second, total RNA was extracted from the mitochondrial and cytosolic fractions. Third, mitochondrial and cytosolic RNA were respectively profiled by microRNA microarray analysis. Last, data were analyzed and normalized.Three independent assays were performed.

Repositories


GEO

GSE24761

ArrayExpress

E-GEOD-24761

BioProject

PRJNA132163

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