Computational protocol: Omega-3 fatty acids correlate with gut microbiome diversity and production of N-carbamylglutamate in middle aged and elderly women

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Protocol publication

[…] We assessed the association between circulating serum levels of DHA, FAW3, LNA, FAW6, total PUFA, total MUFA, total SFA, TotFA by using random intercept linear regression adjusting for age, BMI and family relatedness. Linear regression was also employed to investigate the association between OTUs and DHA adjusting for covariates and multiple testing using false discovery rate (FDR < 0.05). We then calculated the principal coordinates from both weighted and unweighted UniFrac distances, a measure of microbial beta-diversity, as implemented in QIIME to further examine the associations between omega-3 circulating levels and microbiome composition.Finally, we assessed the correlation between DHA circulating levels and faecal metabolites in a sub-analysis of 707 individuals using linear regression adjusting for age, BMI, family relatedness and multiple testing (FDR < 0.05). We then tested the associations between the significant faecal metabolites and the previously identified OTU adjusting also for DHA. […]

Pipeline specifications

Software tools UniFrac, QIIME
Application 16S rRNA-seq analysis
Organisms Homo sapiens
Chemicals Fatty Acids, Fatty Acids, Omega-3