Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Mar 8 2013
Last update date: Jan 12 2017
Access: Public
Taxon: Mus musculus
Dataset link Transcriptional analysis of genes regulating the mitochondrial dNTP pool in muscle cells.

Experimental Protocol


Proliferating mouse C2C12 myoblasts were collected at about 50 percent confluence. Myoblasts were then induced to differentiate in vitro into myotubes that were harvested after 96 hours and further purified to reduce the contribution of mononucleated cells present in the culture. Gene expression in C2C12 myoblasts and myotubes was compared with fully differentiated muscle fibers in vivo. To this aim, the extensor digitorum longus (EDL) and soleus hind limb muscles were isolated from adult CD1 mice. These muscles were selected because they have different metabolic (glycolytic vs. oxidative) and twitching (fast vs. slow) properties. Triplicate total RNA samples were submitted to gene expression profiling.

Repositories


GEO

GSE41877

ArrayExpress

E-GEOD-41877

BioProject

PRJNA178465

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Contact


Gerolamo Lanfranchi

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