|Application:||Gene expression microarray analysis|
|Number of samples:||12|
|Release date:||Mar 8 2013|
|Last update date:||Jan 12 2017|
|Dataset link||Transcriptional analysis of genes regulating the mitochondrial dNTP pool in muscle cells.|
Proliferating mouse C2C12 myoblasts were collected at about 50 percent confluence. Myoblasts were then induced to differentiate in vitro into myotubes that were harvested after 96 hours and further purified to reduce the contribution of mononucleated cells present in the culture. Gene expression in C2C12 myoblasts and myotubes was compared with fully differentiated muscle fibers in vivo. To this aim, the extensor digitorum longus (EDL) and soleus hind limb muscles were isolated from adult CD1 mice. These muscles were selected because they have different metabolic (glycolytic vs. oxidative) and twitching (fast vs. slow) properties. Triplicate total RNA samples were submitted to gene expression profiling.
Citations per year
The Journal of Biological Chemistry