Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 27
Release date: Jul 12 2013
Last update date: Aug 23 2018
Access: Public
Diseases: Down Syndrome, Migraine without Aura
Chemicals: Zinc
Dataset link Translating Dosage Compensation to Trisomy 21

Experimental Protocol


Three independently targeted subclones plus the parental Chr21 trisomic (non-targeted) iPS cell line were grown ± doxycycline (2 µg/ml) for 22 d. Normal male iPS line was also cultured for 22 d and total RNA was extracted with a High Pure RNA extraction kit (Roche) in triplicate for each, processed with a Gene Chip 3’ IVT Express Kit (Affymetrix), and hybridized to Affymetrix human gene expression PrimeView arrays. Array normalization was performed with Affymetrix Expression Console Software with Robust Multichip Analysis (RMA). Probesets with the top 60% of signal values were considered present and “expressed” and were used for all further analysis. In total 9 RNA samples were prepared in triplicate for a total of 27 arrays.

Repositories


GEO

GSE47014

ArrayExpress

E-GEOD-47014

BioProject

PRJNA203276

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Contact


Benjamin Carone
Benjamin R Carone

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