|Application:||Gene expression microarray analysis|
|Number of samples:||27|
|Release date:||Jul 12 2013|
|Last update date:||Aug 23 2018|
|Diseases:||Down Syndrome, Migraine without Aura|
|Dataset link||Translating Dosage Compensation to Trisomy 21|
Three independently targeted subclones plus the parental Chr21 trisomic (non-targeted) iPS cell line were grown ± doxycycline (2 µg/ml) for 22 d. Normal male iPS line was also cultured for 22 d and total RNA was extracted with a High Pure RNA extraction kit (Roche) in triplicate for each, processed with a Gene Chip 3’ IVT Express Kit (Affymetrix), and hybridized to Affymetrix human gene expression PrimeView arrays. Array normalization was performed with Affymetrix Expression Console Software with Robust Multichip Analysis (RMA). Probesets with the top 60% of signal values were considered present and “expressed” and were used for all further analysis. In total 9 RNA samples were prepared in triplicate for a total of 27 arrays.
Benjamin R Carone