The Long Noncoding RNA Lncenc1 Regulates Glycolysis in Naive Mouse ES Cells II

The naive embryonic stem cells (nESCs) display unique characteristics mirroring naive pluripotency in vivo, but the molecular mechanisms underlying the self-renewal of nESCs remain incompletely understood. Here we analyzed stranded transcriptomes in mouse nESCs and epiblast-like cells (EpiLCs), and identified 135 long non-coding RNAs (lncRNAs) preferentially expressed in nESCs. We further investigated the functions of Lncenc1, a highly abundant lncRNA in mouse nESCs. Knockdown or knockout of Lncenc1 in mouse nESCs leads to significantly decreased expression of core pluripotency genes and significant reduction of colony formation capability. Furthermore, depletion of Lncenc1 down-regulates the glycolysis pathway, as indicated by significant decreases of glycolysis genes expression, glucose consumption, lactate production and Seahorse data. Mechanically, Lncenc1 associates with RNA-binding proteins PTBP1 and HNRNPK functionally. Together, we demonstrate that Lncenc1 regulates the glycolysis in mouse nESCs, suggesting novel functions of lncRNAs in linking energy metabolism and pluripotent stem cells.

Dataset features

Information

Specifications

Application:	RNA-seq analysis
Number of samples:	4
Release date:	Aug 1 2018
Last update date:	Nov 21 2018
Access:	Public
Chemicals:	Glucose, Lactic Acid
Dataset link	The Long Noncoding RNA Lncenc1 Regulates Glycolysis in Naive Mouse ES Cells II

Experimental Protocol

RNAseq data in nESCs and Lncenc1 KD nESCs

Repositories

GEO

GSE116462

ENA

SRP151678

BioProject

PRJNA478593

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Contact

Bo Wen