Computational protocol: Identification of trkH, Encoding a Potassium Uptake Protein Required for Francisella tularensis Systemic Dissemination in Mice

Similar protocols

Protocol publication

[…] LVS genome sequence analyses were performed essentially from the KEGG database ( and associated sofware.F. tularensis complete genomes were available at the NCBI entrez Genome project site (,16421,17265,19571,17375,9,18459).The program BLASTN 2.2.22 was used on the nucleotide collection (nr/nt) available at sequence similarity searches were performed using BLASTP against non-redundant protein data bases. Multiple sequence alignment of homologous sequences was carried out using ClustalW .TMHMM server version 2.0 ( was used to perform TMS predictions.” […]

Pipeline specifications

Software tools BLASTN, BLASTP, Clustal W, TMHMM
Databases KEGG
Application Membrane protein analysis
Organisms Francisella tularensis, Mus musculus
Diseases Tularemia, Zoonoses, Mastocytosis, Systemic
Chemicals Potassium