Computational protocol: ROCK Inhibition Facilitates In Vitro Expansion of Glioblastoma Stem-Like Cells

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Protocol publication

[…] Cells were cultured for six days in control media and in media supplemented with either 45 μM Y-27632 or10 μM fasudil. Micrographs were taken (n = 20) of each experimental group at 4X magnification throughout the culture period. The number of spheres was counted in each micrograph. The diameter of each tumorsphere was measured using ImageJ (National Institutes of Health, Bethesda, MD). Statistical analyses were performed in Minitab v16 (Minitab Inc., State College, PA). […]

Pipeline specifications

Software tools ImageJ, Minitab
Application Microscopic phenotype analysis
Diseases Brain Neoplasms, Glioblastoma, Neoplasms