Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Oct 14 2015
Last update date: Aug 13 2018
Access: Public
Diseases: Hepatitis, Hepatitis C, Lymphoproliferative Disorders, Leukemia, B-Cell, Lymphoma, B-Cell
Dataset link Hepatitis C virus up-regulates B-cell receptor signaling: a novel mechanism for HCV-associated B-cell lymphoproliferative disorders

Experimental Protocol


A series of FLAG epitope-tagged NS3/4A truncation and deletion mutants were cloned into a lentiviral-vector CD532, and stable cell lines of SUDHL6-overexpressing FLAG epitope-tagged NS3/4A truncation and deletion mutants generated by lentiviral transduction, selection and maintenance with 0.5 mg/ml puromycin. Ribonucleoprotein immunoprecipitation (RNP IP) using an anti-HuR antibody of control (CTR_S6_532_2_x) and NS3/4A-overexpressing (NS3_S6_8_2_x) SUDHL6-cells generated HuR-IP RNA samples for microarray analysis of differences in HuR binding activity caused by NS3/4A-overexpression. Three independent experiments are reported for each condition.

Repositories


GEO

GSE71757

BioProject

PRJNA292015

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Contact


Kevin Becker

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