|Application:||Gene expression microarray analysis|
|Number of samples:||6|
|Release date:||Oct 14 2015|
|Last update date:||Aug 13 2018|
|Diseases:||Hepatitis, Hepatitis C, Lymphoproliferative Disorders, Leukemia, B-Cell, Lymphoma, B-Cell|
|Dataset link||Hepatitis C virus up-regulates B-cell receptor signaling: a novel mechanism for HCV-associated B-cell lymphoproliferative disorders|
A series of FLAG epitope-tagged NS3/4A truncation and deletion mutants were cloned into a lentiviral-vector CD532, and stable cell lines of SUDHL6-overexpressing FLAG epitope-tagged NS3/4A truncation and deletion mutants generated by lentiviral transduction, selection and maintenance with 0.5 mg/ml puromycin. Ribonucleoprotein immunoprecipitation (RNP IP) using an anti-HuR antibody of control (CTR_S6_532_2_x) and NS3/4A-overexpressing (NS3_S6_8_2_x) SUDHL6-cells generated HuR-IP RNA samples for microarray analysis of differences in HuR binding activity caused by NS3/4A-overexpression. Three independent experiments are reported for each condition.
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