Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Mar 31 2010
Last update date: Aug 15 2018
Access: Public
Chemicals: Chlorophyll, Cysteine, Glutathione, Sulfhydryl Compounds
Dataset link A novel S-sulfocysteine synthase essential for chloroplast function in Arabidopsis thaliana

Experimental Protocol


Using the Affymetrix ATH1 GeneChips, we performed a comparative transcriptomic analysis on leaves of the cs26 and wild type plants under two different photoperiod conditions. Wild type and cs26 mutant plants were grown on soil under a long-day photoperiod (LD) or under a short-day photoperiod (SD). Total RNA was extracted from the leaves of 3-week-old plants grown under identical LD conditions, and from the leaves of 5-week-old plants grown under identical SD conditions. Three biological replicates were performed for each sample and hybridized to the chips. We made two different comparisons to classify the differently expressed genes in the mutant plant: cs26 leaves under LD versus wild-type leaves under LD and cs26 leaves under SD versus wild-type leaves under SD.

Repositories


GEO

GSE19241

ArrayExpress

E-GEOD-19241

BioProject

PRJNA120773

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Contact


Cecilia Gotor