|Application:||Gene expression microarray analysis|
|Number of samples:||20|
|Release date:||May 2 2006|
|Last update date:||Oct 29 2018|
|Diseases:||Dementia, Neurodegenerative Diseases, Diffuse Neurofibrillary Tangles with Calcification|
|Dataset link||Alzheimer’s disease: neurofibrillary tangles (Rogers-3U24NS043571-01S1)|
First use laser capture microdissection to select 1000 neurons bearing neurofibrillary tangles and 1000 normal neurons from the layer 2 stellate island neurons of the entorhinal cortex of 10 mid-stage AD cases. Second, to isolate the RNA from these captured neurons, perform double round linear amplification and hybridize the labeled cRNA to affymetrix U133A arrays. Third, to use paired, permutational t-tests to analyze the microarray data to select tangle-specific differences in gene expression. For the purposes of submitting this proposal, a value of 1 ug of RNA was entered due to web site constraints on values placed in that field. However, all samples are from LCM captured cells and the actual RNA yield is likely closer to 100 pg.