Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Nov 7 2012
Last update date: Jun 12 2017
Access: Public
Chemicals: Amino Acids, Glucosinolates, Methionine
Dataset link Partial deficiency of isoleucine impairs alters transcript levels of the genes involved in branched-chain amino acid and glucosinolate metabolism in Arabidopsis

Experimental Protocol


Total RNAs were isolated from three replicate samples of the root tissues of 8-day-old wild type (w) and lib mutant (m)seedlings grown on 1/2 MS medium and hybridized on Affymetrix ATH1 microarrays. The changes of gene expression in the lib mutant (m) was compared with the wild type (w). The corrected P value of <0.05 was used to select the genes for comparison, and only those genes whose expression was two-fold higher or lower in lib than in the WT were used for analysis.

Repositories


GEO

GSE42060

ArrayExpress

E-GEOD-42060

BioProject

PRJNA179046

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Contact


Dong Liu

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