Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 48
Release date: Mar 23 2016
Last update date: Jun 22 2016
Access: Public
Dataset link Boolean modeling reveals the necessity of transcriptional regulation for bistablity in PC12 Cell Differentiation

Experimental Protocol


To eludicate the transcriptome response of PC12 cells time-resolved gene expression data of NGF or EGF simulated PC12 were recorded at t = [0.5h 1h, 2h, 3h, 4h, 5h, 6h, 8h, 12h, 24h, 48h] for NGF and at t=[1h, 2h, 3h, 4h, 6h, 8h, 12h, 24h]. Control time points of unstimulated PC12 cells were taken at t=[0h, 2h, 4h, 6h, 8h, 24h, 48h]. Additionally, the MEK-inhibitor UO126 was added together with NGF and the gene response was measured at t=[1h, 2h, 4h, 6h, 8h, 12h, 24h, 48h] and for UO126 alone at t=[1h, 2h, 4h, 8h, 12h, 48h]. Total RNA was isolated, labeled and hybridized to an Illumina ratRef-12 bead array (Illumina, San Diego, CA, USA) according to the manufacturer’s protocol.

Repositories


GEO

GSE74327

ArrayExpress

E-GEOD-74327

BioProject

PRJNA299684

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Contact


Hauke Busch

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