Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Dec 23 2016
Last update date: Feb 21 2018
Access: Public
Diseases: Leukemia
Dataset link Azacitidine mitigates GvHD via its differential effects on expansion of nTregs in vivo.

Experimental Protocol


n=3 per group nTregs and AzaC generated Tregs, nTregs were purified from the spleens of B6.Foxp3GFP mice and Treg depleted T cells were obtained from B6. Foxp3GFP x B6.CAGDSRED. T Cells were co-cultured at a 1:10 ratio of nTreg to Teff and were activated for 2 days in the presence of anti-CD3/CD28 beads (bead:cell 1:1; Invitrogen) and Xcyte medium supplemented with L-glutamine (4mM), penicillin (100 U/mL), streptomycin (100 µg/mL), and human recombinant IL-2 (hIL-2; 500 U/mL). The activated T cells were incubated in the presence of AzaC (1µM) (Sigma-Aldrich) or PBS for an additional 2 days. Cells were sorted using FACS Aria II (BD) to isolate nTreg (CD4+DSRED-FOXP3GFP+), CD4+ Teff (CD4+DSRed+FOXP3GFP-), CD8+ Teff (CD8+DSRed+FOXP3GFP-).

Repositories


GEO

GSE92841

BioProject

PRJNA358602

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Contact


John DiPersio