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[…] hus proliferate in contaminated environments. Although genes conferring metal resistance or related to hydrocarbon degradation were identified in previously reported Psychrobacter genome sequences, the genome here analyzed is the one that comprises a more diverse repertoire of these genetic determinants. More studies are needed to confirm a future usefulness of this strain for bioremediation purposes., Genome sequence of Psychrobacter sp. ENNN9_III was obtained by using a combined approach with the SOLiD 5500xl (Life Technologies) and the Ion Torrent PGM (Life Technologies) platforms. Both sequencing approaches were based on paired-ended libraries. Quality of raw reads was assessed with the FastQC software (http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) and the bases with quality value bellow Phred 20 were removed., Draft genome was assembled using the packages Velvet , MIRA and SPAdes . CISA and Lasergene v11 Suite (DNAStar) software were used to reduce the number of contigs. Subsequently, the Mauve software was applied to order the draft genome by using the genomes of Psychrobacter cryohalolentis and Psychrobacter arcticus 273-4 as references., The Gepard software was used for synteny analysis . The automatic annotation was used by RAST — Rapid Annotations using Subsystems Technology . The rRNA and tRNA were predicted by RNAmmer and tRNAscan-Se , respectively. Predicted coding sequences (CDSs) were annotated using Blast2GO . Functional classification of CDSs was performed based on Gene Ontology databases., The tree was generated based on the 16S rRNA gene sequences using the Maximum Likelihood method based on the General Time Reversible model in MEGA v6 software . Bootstrap values greater than 50% are shown above the node of each main group. Sequences of the strains Moraxella catarrhalis (AF005185), Moraxella lacunata (AF005160), Acinetobacter calcoaceticus strain NCCB 22016 (NR_042387) and Acinetobacter calcoaceticus strain HPC253 (AY346313) were used as outgroup. The scale bar corresponds to the nucleotide substitution rate., The authors have declared that no competing interests exist., This work wa […]

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