Dataset features

Specifications


Dataset type: Expression profiling by array, transcription profiling by array, Transcriptome or Gene expression
Number of samples: 44
Release date: Oct 17 2013
Last update date: Nov 9 2017
Access: Public
Genes: HIST1H2BA, Hist1h2ba
Taxon: Mus musculus
Dataset link Chromatin-to-nucleoprotamine transition is controlled by the histone H2B variant TH2B [Illumina]

Experimental Protocol


Total RNA were obtained from testes from meiotic (spermatocytes) and post-meiotic (round spermatids) male germ cells from adult wt and Th2b+/tag mice (experiment 1: 6 biological replicates for each condition) and adult wt and th2bkd mice (experiment 2: 5 biological replicates for each condition). In each experiment, 5 or 6 replicates of each genotype and each cell type were used. Th2b+/tag mice expressed TH2B C-terminally fused to three consecutive affinity tags: His, Flag and Ha. Th2bkd mice did not express any TH2B.

Repositories


GEO

GSE46136

ArrayExpress

E-GEOD-46136

BioProject

PRJNA197337

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Contact


Sophie Rousseaux