Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 96
Release date: Sep 6 2018
Last update date: Sep 6 2018
Access: Public
Dataset link nCounter analysis of mRNAs associated with occupational benzene exposure using a Nanostring custom assay

Experimental Protocol


We used the nCounter platform and a custom assay to validate altered expression of 30 mRNAs in total RNA from peripheral blood mononuclear cells from 57 subjects occupationally exposed to benzene (<1 to ≥5 ppm) and 33 non-occupationally-exposed controls. A custom probeset comprising genes previously detected as differentially expressed by microarray and mRNA-Seq and 3 reference genes was designed. Six randonly selected technical replicates were included in the assay.

Repositories


GEO

GSE119533

BioProject

PRJNA489476

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Contact


Cliona McHale