Computational protocol: Ezrin regulates microvillus morphogenesis by promoting distinct activities of Eps8 proteins

Similar protocols

Protocol publication

[…] Cells were fixed with paraformaldehyde (4%) and processed for immunofluorescence. Images were taken with a Nikon 90i upright microscope equipped with PIFOC objective stepper and a 100×/1.4 numerical aperture Plan Apochromat objective (Nikon, Champigny sur Marne, France). Slices were acquired along the z-axis every 0.2 μm. Deconvolution was done by the MetaMorph module (Roper Scientific, Sarasota, FL) using the Meinel algorithm. Image processing and analyses were performed with ImageJ (National Institutes of Health, Bethesda, MD; ). […]

Pipeline specifications

Software tools MetaMorph, ImageJ
Applications cryo-EM, Microscopic phenotype analysis