Computational protocol: Identification and Functional Characterization of a Tonoplast Dicarboxylate Transporter in Tomato (Solanum lycopersicum)

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Protocol publication

[…] Sequence alignment of SlTDT and tonoplast dicarboxylate transporters from other organisms was performed using ClustalX (version 2.1) software () with default multiple parameters and PAM series protein weight matrix. The alignment was then imported into MEGA (version 5.2) software and the phylogenetic tree was constructed by the neighbor-joining statistical method with 1000 bootstrap replicates. […]

Pipeline specifications

Software tools Clustal W, MEGA
Application Phylogenetics
Organisms Solanum lycopersicum