Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 8
Release date: Nov 30 2012
Last update date: Apr 19 2017
Access: Public
Genes: PYR1, PYL2, PYL4, PYL5, RCAR3
Taxon: Arabidopsis thaliana
Dataset link PYR/PYL receptors play a major role for regulation of transcriptional response to abscisic acid

Experimental Protocol


Four biological replicates were generated for the 3 genotypes, pyr1pyl1pyl2pyl4pyl5pyl8 sextuple mutant, snrk2.2/2.3/2.6 triple mutant, and Col-0. All the samples were treated with ABA for 3 hours before harvesting. The four Col-0 samples were mix to create an unique reference Col-0 sample. Two comparasion were made, fisrt one, PYR/PYL sextuple mutant versus Col-0 reference sample and second one, SnRK2 triple mutant versus Col-0 reference sample. In each comparasion four biological replicates were made. Replicas number 1 and 2 were labeled with Cy5 for the mutant sample and Cy3 for the Col-0 reference sample, while the other two replicas,#3 and #4, were reversed-labeled.

Repositories


GEO

GSE36692

ArrayExpress

E-GEOD-36692

BioProject

PRJNA153613

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Contact


Francisco Vera-Sirera
Francisco Vera-Sirera