|Application:||Gene expression microarray analysis|
|Number of samples:||8|
|Release date:||Nov 30 2012|
|Last update date:||Apr 19 2017|
|Genes:||PYR1, PYL2, PYL4, PYL5, RCAR3|
|Dataset link||PYR/PYL receptors play a major role for regulation of transcriptional response to abscisic acid|
Four biological replicates were generated for the 3 genotypes, pyr1pyl1pyl2pyl4pyl5pyl8 sextuple mutant, snrk2.2/2.3/2.6 triple mutant, and Col-0. All the samples were treated with ABA for 3 hours before harvesting. The four Col-0 samples were mix to create an unique reference Col-0 sample. Two comparasion were made, fisrt one, PYR/PYL sextuple mutant versus Col-0 reference sample and second one, SnRK2 triple mutant versus Col-0 reference sample. In each comparasion four biological replicates were made. Replicas number 1 and 2 were labeled with Cy5 for the mutant sample and Cy3 for the Col-0 reference sample, while the other two replicas,#3 and #4, were reversed-labeled.