Computational protocol: Dominant Rio1 kinase/ATPase catalytic mutant induces trapping of late pre-40S biogenesis factors in 80S-like ribosomes

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Protocol publication

[…] Data were collected at 100K at the NE-CAT beamline (24-ID) at the Advanced Photon Source, ANL, Argonne, IL, USA. All data were indexed, integrated and scaled using HKL2000 program (). The crystals belonged to the P3221space group with unit cell dimensions of a = b = 78.76, c = 110.57, and one molecule per asymmetric unit. The structure was solved by a combination of molecular replacement using the structure of Archaeoglobus fulgidus Rio1 (PDB: 1ZP9) and single-wavelength anomalous dispersion using seleno-methionine-modified crystals, using PHASER in PHENIX (). The structure was rebuilt using COOT and refined in PHENIX (,). Data collection and refinement statistics are provided in Table . […]

Pipeline specifications

Software tools PHENIX, Coot
Application Protein structure analysis
Organisms Homo sapiens, Saccharomyces cerevisiae
Chemicals Adenosine Triphosphate