Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 26
Release date: Aug 31 2017
Last update date: Nov 29 2018
Access: Public
Diseases: Encephalitis, Arbovirus
Mutations: p|SUB|A|82|V
Dataset link Ebola virus glycoprotein variant with increased infectivity for human cells dominated the 2013-2016 outbreak

Experimental Protocol


We generated stocks of lentiviral vector bearing one the following three M-EBOV GPs: founder, A82V, and A82V/T230A. These viral stocks were used to challenge MDDCs from two healthy, anonymous human donors. Stimulated MDDCs were harvested at 1, 2, 4, and 6 hours after viral addition. Gene expression in M-EBOV GP challenged MDDCs was compared to a unstimulated control.

Repositories


GEO

GSE84865

ENA

SRP079916

BioProject

PRJNA335391

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Contact


Alper Kucukural