Computational protocol: Nanoscale visualization of functional adhesion/excitability nodes at the intercalated disc

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Protocol publication

[…] Fluorescent beads (0.1 μm diameter, Life Technologies) were adsorbed on a coverglass surface at a low density. A z-stack image was generated by acquiring images at 10 nm z-intervals, ranging from −500 to 500 nm considering 0 when the beads were in focus. A 3D calibration table with the ‘PSF Width minus Height' values over the z-position was created using the plugin QuickPALM on ImageJ (). […]

Pipeline specifications

Software tools QuickPALM, ImageJ
Applications Super-resolution imaging, Microscopic phenotype analysis
Organisms Homo sapiens
Diseases Cardiomyopathies
Chemicals Sodium