Dataset features

Specifications


Application: BS-seq analysis
Number of samples: 10
Release date: Mar 3 2015
Last update date: Dec 9 2016
Access: Public
Diseases: Tumor Lysis Syndrome
Dataset link Direct but No Transgenerational Effects of Decitabine and Vorinostat on Male Fertility

Experimental Protocol


We studied genome-wide DNA methylation changes in decitabine treated mice and their F3-generation. For this, we treated male C57BL/6 mice (seven weeks old) for seven weeks (intraperitoneal, three times per week) with the DNMT inhibitor decitabine (5-aza-2'-deoxycytidine, 0.1 µg/g, n = 17) or with vehicle (7.5 % dimethyl sulfoxide in phosphate buffered saline, n = 16). The dose of decitabine per treatment was chosen according to the clinical application regimens in humans and refer to previous mice studies using this drug (Kelly et al, 2003; Oakes et al, 2007). For the investigation of transgenerational effects, 10 treated and untreated male mice were mated with four untreated female C57BL/6 mice each. The offspring was considered as F1-generation. The F2- and F3-generations were obtained by an identical mating scheme. Thus, only the P-generation males were treated with decitabine or control vehicle, the F1-, F2- and F3-generations were not subjected to any treatment. During this study, all mice were housed at 24 °C on a 12-h light, 12-h dark cycle and provided with food and tap-water ad libitum.

Repositories


GEO

GSE59575

ArrayExpress

E-GEOD-59575

ENA

SRP044642

BioProject

PRJNA255653

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Contact


Christian Rohde

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