|Application:||RNA-seq analysis, sRNA-seq analysis|
|Number of samples:||3|
|Release date:||Aug 4 2012|
|Last update date:||Jan 5 2016|
|Dataset link||Arabidopsis AGO1 and AGO10 bound sRNAs|
Homozygous T2 progeny of complemented plants expressing ago10-3;PAGO10-HF-AGO10 and ago1;PAGO1-HF-AGO1 were used for preparation of AGO complexes. Flower samples including floral buds, open flowers, and newly set siliques (1-2 day old) were collected for protein extraction and isolation of dual-tagged AGO complexes using a two-step affinity purification. The isolated AGO complexes were divided into two parts, one aliquot was used for sRNA extraction with Trizol reagent, whereas the other part was used for monitoring protein purity by Gelcode blue staining and western blot using a monoclonal anti-Flag antibody (Sigma) as previous described.