Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 15
Release date: Dec 11 2013
Last update date: Jul 26 2018
Access: Public
Diseases: Prostatic Neoplasms, Retinitis Pigmentosa
Dataset link Hypermethylation of GABRE~miR-452~miR-224 in prostate cancer predicts biochemical recurrence after radical prostatectomy

Experimental Protocol


Affymetrix GeneChip Human Gene 1.0 ST Arrays were used for whole-genome transcriptional profiling of DU145 and PC3 cells at 48 hours post-transfection with either miR-224, miR-452, or scrambled miRNA mimics, or untransfected. All experiments were performed in duplicate. Transcript expression levels were determined after RMA16 normalization in GeneSpringGX 11.0 software (Agilent). PC3 and DU145 arrays were normalized separately. DU145 48 t Scr2a were excluded from the study because of bad performance of this microarray.

Repositories


GEO

GSE51053

ArrayExpress

E-GEOD-51053

BioProject

PRJNA219828

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Contact


Kasper Thorsen