Computational protocol: Association of the DYX1C1 Dyslexia Susceptibility Gene with Orthography in the Chinese Population

Similar protocols

Protocol publication

[…] The PLINK program version 1.07 was used to carry out the Hardy-Weinberg Equilibrium (HWE) test of all three SNPs and to examine the association between SNPs or haplotypes and the quantitative measures. In line with previous studies , , additive models were considered and were evaluated against the null hypothesis of no association. As the three SNPs were low in linkage disequilibrium (LD) (, r2 ranged from 0.002 to 0.054), only the widely reported haplotype spanning rs57809907/rs3743205 , , was analyzed in the present study. Linkage disequilibrium of the SNPs was estimated using Haploview Version 4.2 . The SPSS 13.0 program was used to perform descriptive statistics and Pearson correlations. SNP association was performed with Chinese character reading and Chinese character dictation in the first step. SNPs that showed significant association in the first step were then further explored with visual-related measures and orthographic judgment. Finally, SNPs that showed significant association with orthographic judgments were then tested for associations with each of the four orthographic conditions. In all analyses, both age and sex were specified as covariates. We corrected for the number of SNPs, which yielded a significance level of 0.0167 after Bonferroni correction. Since all quantitative phenotypes were highly correlated (see ), we did not correct for multiple phenotypes tested. […]

Pipeline specifications

Software tools PLINK, Haploview, SPSS
Applications Miscellaneous, GWAS
Organisms Homo sapiens