|Application:||Gene expression microarray analysis|
|Number of samples:||14|
|Release date:||Oct 23 2008|
|Last update date:||Mar 17 2012|
|Dataset link||Transcriptional profiles of Staphylococcus aureus strains in the presence of the lantibiotic mersacidin.|
In this experiment, the transcriptomic response of S. aureus was assayed via microarray analysis. To this end, S. aureus strains SA137/93A [mersacidin MIC 35 mg/L] and SA137/93G [mersacidin MIC 30 mg/L] were grown without or with mersacidin [16 mg/L] to determine the genes being induced or repressed by the lantibiotic. SA137/93G was additionally grown in the presence of 4 mg/L mersacidin. Furthermore, the highly susceptible S. aureus strain SG511 [mersacidin MIC 1 mg/L] was also grown without or with mersacidin [1 mg/L]. Each experiment was performed 4 times including a dye swap resulting in four chips per competitive comparison to increase reproducibility. Only strain SA137/93G incubated with 0.2 x MIC of mersacidin was reproduced in duplicate. All hybridizations were done with equal amounts of cDNA probes displaying similar picomoles of incorporated dye.
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