Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Oct 22 2009
Last update date: Oct 29 2018
Access: Public
Diseases: Deficiency Diseases
Chemicals: Cysteine, Glutamic Acid
Dataset link UV- or oxidized lipid treated dermal skin fibroblasts

Experimental Protocol


we profiled global mRNA expression levels in human dermal fibroblasts that had been treated with either UVA-1 or oxidized lipids. To investigate the effect of oxidized phospholipids on gene regulation, we used two preparations, which differed in their degree of oxidation; the minimally oxidized UV-PAPC resulting from UVA-1 irradiation of PAPC, and air-oxidized PAPC (OxPAPC), which represents the full spectrum of oxidation products (Gruber 07) (Reis et al., 2005). We irradiated dermal fibroblasts with UVA-1 (40J/cm²) or treated them with UV-PAPC, OxPAPC or native PAPC (100µg/ml each). We analyzed global gene expression four hours after stimulation with gene arrays (Affymetrix U133A Plus 2.0 Gene Chips).

Repositories


GEO

GSE13606

ArrayExpress

E-GEOD-13606

BioProject

PRJNA110627

Download


Contact


Martin Bilban