Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 9
Release date: May 29 2013
Last update date: Oct 29 2018
Access: Public
Diseases: Breast Neoplasms, Cardiovascular Diseases, Colonic Neoplasms, Neoplasms
Chemicals: Polyphenols
Dataset link Comparison of gene expression profiles of HT29 cells treated with Instant Caffeinated Coffee or Caffeic Acid versus control.

Experimental Protocol


The aim of our study was to evaluate, by using whole genome microarrays, the effects of one cup of coffee, either regular caffeinated coffee or a coffee polyphenol, such as caffeic acid, on HT-29 gene expression, Three experimental approaches were conducted to assess the effects of coffee on HT29 cells. I) Incubation with non cytotoxic concentrations of ICC (7 µg/mL) for 24h. (Group ICC); II) Incubation with non cytotoxic concentrations of CA (1.68 µg/mL) for 24h. (Group CA); III) non treatment of HT-29 to refered as a control (Group CNT). Triplicate samples were hybridized for each experimental condition (9 samples in total). The samples provided were analyzed using the specific software GeneSpring GX.

Repositories


GEO

GSE35382

ArrayExpress

E-GEOD-35382

BioProject

PRJNA152621

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Contact


Carlos Ciudad
carlos j ciudad

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