Dataset features


Application: Gene expression microarray analysis
Number of samples: 48
Release date: Mar 29 2013
Last update date: Jul 19 2017
Access: Public
Diseases: Influenza, Human, Tumor Virus Infections
Computational protocol: Agilent Feature Extraction, GeneSpring GX, IPA, Realtime PCR
Dataset link Protective efficacy of orally administered, heat-killed Lactobacillus pentosus b240 against influenza A virus

Experimental Protocol

Six-week-old female BALB/c mice were used in the study. Oral administration of b240 was initiated in mice at six weeks of age. Mice were orally administered heat-killed Lactobacillus pentosus b240 every day at a dose of 10 mg/mouse in 200 μl of buffered saline for 5 weeks. The control group received saline. To investigate the effects of oral administration of b240 on host immune responses to CA04 virus infection, 9 mice per group were infected with 10 MLD50 of CA04 virus on day 21 post-b240 administration. Three mice per group were euthanized on days 1, 3, and 6 post-infection and their lungs were collected. To investigate the immune responses induced by oral administration of b240 in the lungs of uninfected mice, 15 mice per group were mock-infected with PBS on day 21 post-b240 administration. Three mice per group were euthanized on days 14, 21, 22, 24, and 27 post-b240 administration (-7, 0, 1, 3, and 6 days post-mock infection) and their lungs were collected. These lung tissues were subjected to microarray analysis (three biological replicates per each group).










Ryo Takano

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