|Application:||Gene expression microarray analysis|
|Number of samples:||58|
|Release date:||May 19 2010|
|Last update date:||Jan 18 2013|
|Diseases:||Idiopathic Pulmonary Fibrosis|
|Dataset link||Genomic Phenotype Of Non-Cultured IPF Fibroblasts|
58 samples of total RNA isolated from 12 lungs of patients with end-stage idiopathic pulmonary fibrosis and 6 donors of normal lungs (controls) who were designated brain dead, non-diseased donors whose lungs failed criteria for transplantation and who were organ donors for research. RNA extraction followed the Qiagen RNeasy Kit using QIshredder columns for shredding of DNA contiminants. Experimental/control samples were amplified amino-allylated RNA labeled with Cy5 and Stratagene Reference RNA was amplified and amino-allylated and labeled with Cy3. Amplification was one round using Ambion MessageAmp II kit with amino-allylated UTP according to the protocol of the Duke University Institute for Genome Sciences and Policy. Amplification and amino-allylation of the Stratagene Reference RNA and Hybridization of Reference with patient samples and controls was done by the Duke Institute for Genomic Sciences and Policy.
Margaret Claire Emblom-Callahan