|Application:||Gene expression microarray analysis|
|Number of samples:||93|
|Release date:||Aug 12 2011|
|Last update date:||Mar 23 2012|
|Diseases:||Lung Diseases, Fungal|
|Dataset link||Experimental annotation of Histoplasma capsulatum transcribed regions using high-resolution tiling arrays|
The non-redundant genome sequence of Histoplasma capsulatum G217B was tiled over a set of 93 CombiMatrix arrays, which were then hybridized with labeled cDNA samples from yeast-form (red channel) or mycelial-form (green channel) Histoplasma. Due to low yields from the mycelial samples, only the red channel intensities were analyzed (and the red foreground intensities are, therefore, reported in the VALUE column for each sample). Rather than normalizing intensities across arrays, each probe was evaluated as detected or undetected relative to the negative control intensities on the corresponding array, and the density of detected probes as a function of genome position was used for the remaining analysis.