Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 93
Release date: Aug 12 2011
Last update date: Mar 23 2012
Access: Public
Diseases: Lung Diseases, Fungal
Dataset link Experimental annotation of Histoplasma capsulatum transcribed regions using high-resolution tiling arrays

Experimental Protocol


The non-redundant genome sequence of Histoplasma capsulatum G217B was tiled over a set of 93 CombiMatrix arrays, which were then hybridized with labeled cDNA samples from yeast-form (red channel) or mycelial-form (green channel) Histoplasma. Due to low yields from the mycelial samples, only the red channel intensities were analyzed (and the red foreground intensities are, therefore, reported in the VALUE column for each sample). Rather than normalizing intensities across arrays, each probe was evaluated as detected or undetected relative to the negative control intensities on the corresponding array, and the density of detected probes as a function of genome position was used for the remaining analysis.

Repositories


GEO

GSE31155

ArrayExpress

E-GEOD-31155

BioProject

PRJNA144821

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Contact


Mark Voorhies