Computational protocol: Transvection Is Common Throughout the Drosophila Genome

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Protocol publication

[…] Tissues were dissected in PBS and fixed in 4% formaldehyde in PBS (pH 7.0) for 45–60 min, then rinsed in PBS-TX [phosphate buffered saline (pH 7.2) with 1% Triton X-100]. Antibody staining was performed as described by ) using the following antibodies: 1:1000 dilution of rabbit anti-GFP (Invitrogen), 1:500 rat antimouse CD8a (Invitrogen), 1:500 Alexa Fluor 488 conjugated goat antirabbit (Invitrogen), 1:500 Texas Red conjugated Donkey antirat IgG (Jackson ImmunoResearch Laboratories).Imaging was performed on a Zeiss LSM 510 confocal microscope and Z stacks were analyzed using ImageJ (http://rsbweb.nih.gov/nih-image) and Vaa3D (). ImageJ was used for all postprocessing of images. […]

Pipeline specifications

Software tools ImageJ, Vaa3D
Applications Laser scanning microscopy, Microscopic phenotype analysis
Organisms Drosophila melanogaster