Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 721
Release date: Oct 18 2018
Last update date: Nov 30 2018
Access: Public
Taxon: Mus musculus
Dataset link A peninsular structure coordinates asynchronous differentiation with morphogenesis to generate pancreatic islets

Experimental Protocol


Single-cell suspensions were prepared from pancreata of Neurogenin 3-eGFP mouse embryos sacrificed at different days of embryonic development. Single eGFP-positive cells were FACS-sorted into 96-well plates, and single-cell cDNA was prepared using the SMART-seq protocol. Single-cell sequencing libraries were generated using the Nextera XT DNA library preparation kit and sequenced on an Illumina HiSeq sequencer. Reads were aligned to the mouse reference genome build mm10 with TopHat, and single-cell gene expression profiles were computed using Cufflinks.

Repositories


GEO

GSE121416

ENA

SRP166015

BioProject

PRJNA497287

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Contact


Raghav Chawla