Dataset features

Specifications


Application: RNA-seq analysis, sRNA-seq analysis
Number of samples: 4
Release date: Dec 20 2017
Last update date: Jul 26 2018
Access: Public
Genes: MIR21, MIRLET7I, ATG4A, MIR144, YBX1
Taxon: Danio rerio
Dataset link Identification of a specific 13-miRNA expression signature during follicle activation in Zebrafish

Experimental Protocol


To identify differentially expressed miRNAs and its potential downstream targets during follicle activation of zebrafish, we carried out transcriptomic profiling by both small and regular RNA-sequencing. By intersecting gene lists of the online predicted targets and RNA-seq derived differentially expressed transcripts with a reciprocal expression pattern of miRNAs, we shortlisted 6 pairs of miRNA::target gene for validation using luciferase reporter assay.

Repositories


GEO

GSE92639

ENA

SRP095411

BioProject

PRJNA358209

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Contact


Mingan Sun