Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 15
Release date: May 18 2015
Last update date: Aug 9 2018
Access: Public
Genes: GLI2, IFNG
Taxon: Homo sapiens
Dataset link The transcriptomic responses of THP1 human monocyte-like cells expressing SP110b to interferon gamma stimulation

Experimental Protocol


We sought to identify genetic determinants associated with IFNγ-induced cell death and regulated by SP110b. To that end, THP1 human monocyte-like cells that could be induced by doxycycline (Dox) to over-express SP110b (THP1-SP110b) were generated and 5 experimental groups of THP1-SP110b cells were harvested for RNA extraction and hybridization on Affymetrix microarrays. The 5 groups are as follows: untreated THP1-SP110b cells as control (CON), cells treated with IFNγ for 2 days (IFN_2D), cells treated with Dox plus IFNγ for 2 days (DoxIFN_2D), cells treated with IFNγ for 4 days (IFN_4D), and cells treated with Dox plus IFNγ for 4 days (DoxIFN_4D).

Repositories


GEO

GSE58096

ArrayExpress

E-GEOD-58096

BioProject

PRJNA248895

Download