Computational protocol: Molecular Characterization of FT and FD Homologs from Eriobotrya deflexa Nakai forma koshunensis

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Protocol publication

[…] The full-length coding sequences of EdFT, EdFD1, and EdFD2 were amplified from the cDNA prepared from loquat leaves using Phusion DNA Polymerase (Thermo, USA). Subsequently, the PCR products were cloned into pGEM-T easy vector (Promega, USA). The primers used for gene cloning were listed in Supplementary Table . Amino acid sequences were aligned using ClustalX and BioEdit. Phylogenetic analyses were performed using MEGA by the Neighbor-Joining (N-J) method with 1000 bootstrap replications. The identity of the nucleotide and amino acid between EdFD1 and EdFD2 was analyzed using DNAMAN V6.0. The sequences of the EdFT and two EdFD genes was deposited in GenBank, the accession numbers are KU319433 (EdFT), KU319434 (EdFD1), and KU319435 (EdFD2). […]

Pipeline specifications

Software tools Clustal W, BioEdit, DNAMAN
Application Phylogenetics
Organisms Arabidopsis thaliana
Diseases Dysautonomia, Familial