|Number of samples:||2|
|Release date:||Sep 17 2018|
|Last update date:||Oct 23 2018|
|Dataset link||The APT complex is involved in non-coding RNA transcription and is distinct from CPF [PAR-CLIP]|
In this study, we used photoactivatable ribonucleoside-enhanced crosslinking and immuno-precipitation (PAR-CLIP) and ChIP-seq to map direct protein-RNA interactions and protein-DNA interactions, respectively, in vivo. We applied these methods to subunits of the CPF and APT complexes of the yeast Saccharomyces cerevisiae. Both ChIP and PAR-CLIP occupancy profiles suggested that the APT defining subunit Syc1 is preferentially associated with sn/snoRNA genes and transcripts. Thus, we also investigated RNA synthesis in vivo using metabolic labeling with 4-thiouracil, followed by sequencing of the newly synthesized, labeled RNA (4tU-seq) and compared syc1 knock-out cells to wild-type.