Computational protocol: Effects of Nitrogen Addition on the Drought Susceptibility of the Leymus chinensis Meadow Ecosystem Vary with Drought Duration

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Protocol publication

[…] Data were tested for normality using a Kolmogorov–Smirnov test and for variance homogeneity using Levene’s test. The effects of N addition, drought and measuring time on A, gs, Tr, GEP, ER, NEE, ET, and WUE were analyzed using a repeated-measures analysis of variance (ANOVA), with N addition and drought as the between-subjects factors and measuring time as the within-subject factor. Comparisons of the means of response variables related to treatments (C, D, N, and DN) in each measurement campaign were analyzed with an ANOVA combined with Tukey’s post hoc test. A paired sample t-test was used to assess differences in the change in leaf, community and ecosystem response variables induced by drought (Impact-D) between the fertilized and unfertilized plots. A linear regression analysis was used to examine dependence of the A, gs, and Tr on the soil water content as well as relationships between the GEP and NEE and aboveground phytomass. All statistical analyses were conducted with the SPSS 18.0 software (SPSS Inc., Chicago, IL, United States). Graphs were generated using the SigmaPlot 12.5 software (Systat Software Inc., San Jose, CA, United States). […]

Pipeline specifications

Software tools SPSS, SigmaPlot
Application Miscellaneous
Organisms Uroteuthis chinensis
Chemicals Carbon, Nitrogen