Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Jun 1 2012
Last update date: Mar 26 2013
Access: Public
Dataset link GS003: Tight cooperation between Mot1p and NC2β in regulating genome-wide transcription, repression of transcription following heat shock induction and genetic interaction with SAGA.

Experimental Protocol


In this study 3 S. cerevisiae strains were grown with and without addition of rapamycin. For each experimental factor two independent colonies were inoculated in Complete Synthetic Medium (CSM) with 2% glucose. Overnight cultures were diluted to 0.3 OD600 in 50 ml medium and grown to 1 OD600. Cultures were then grown for 60 minutes in the absence or presence of rapamycin at 1ug/ml (LC laboratories). Cells were harvested by centrifugation at 4000 rpm for 3 minutes and were frozen in liquid nitrogen. Amplified cRNA samples were labeled with either cy3 or cy5 and put on microarray together with an oppositely labeled common reference sample consisting of cRNA of untreated wildtype strain.

Repositories


GEO

GSE30148

ArrayExpress

E-GEOD-30148

BioProject

PRJNA143855

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Contact


Marian Groot Koerkamp

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