Dataset features

Specifications


Dataset type: Expression profiling by RT-PCR, transcription profiling by RT-PCR, Transcriptome or Gene expression
Number of samples: 2
Release date: Apr 15 2015
Last update date: Oct 29 2015
Access: Public
Diseases: Prehypertension
Dataset link Altered neurotrophin expression profiles in the nucleus of the solitary tract of spontaneously hypertensive rats

Experimental Protocol


The NTS was dissected from the brain of 6 SHRs and 6 WKY rats and the total RNA was extracted. In both groups of rats (SHRs & WKY rats, n = 6 each), a total of 2 ug mRNA extracts from each NTS were pooled together, treated with RNase-free DNAse I (Invitrogen Life technologies) to remove any genomic contamination, and further purified using the RNeasy mini kit (Qiagen) according to the manufacturer’s instructions. Reverse transcription was subsequently performed on 1 ug total RNA using SuperArray’s RT2 First Strand Kit (SABiosciences); the resulting cDNA was submitted for real-time quantitative PCR reactions on RT2 ProfilerTM PCR array plates using Superarray RT2 SYBR Green qPCR Master Mix (SAbiosciences) and iCycler iQ thermal cycler (Bio-rad), following the manufacturer’s instructions. The experiment was performed in duplicate in each group.

Repositories


GEO

GSE67874

ArrayExpress

E-GEOD-67874

BioProject

PRJNA281108

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Contact


Sabine Gouraud