Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Feb 15 2008
Last update date: Aug 28 2018
Access: Public
Chemicals: Sirolimus
Genes: AKT1, CD4, FOXP3, MTOR
Dataset link AKT regulates de novo induction of Foxp3

Experimental Protocol


All gene expression profiles were obtained from highly purified T cell populations sorted by flow cytometry. To reduce variability, cells from multiple mice were pooled for sorting, and three replicates were generated for all groups. RNA from 0.5-2.5 x 105 cells was amplified, labeled, and hybridized to Affymetrix M430v2 microarrays. Raw data were preprocessed with the RMA algorithm in GenePattern, and averaged expression values were used for analysis.

Repositories


GEO

GSE7596

ArrayExpress

E-GEOD-7596

BioProject

PRJNA100163

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Contact


CBDM Lab
CBDM Z. Lab

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